Contact an Account ManagerSeparation of water soluble vitamins on NUCLEOSHELL HILIC
Summary
Type: HPLC
Application #: 125450
Catalog#: 763334.40 - MN HPLC Column, Nucleoshell Hilic, 90A, 2.7um, 4x100mm
Detection:
Conditions: Eluent A: acetonitrile - 100 mmol/L ammonium formate, pH 3.2 (90:10, v/v)
Eluent B: water
Gradient: 4 % B (1 min) -> 20 % B in 1.6 min, hold 20 % B for 0.7 min
Flow rate: 2.0 mL/min
Temperature: 25 °C
Pressure: 218 bar
Injection volume: 1.0 µL
Authors: HPLC department
Source: Macherey-Nagel, Germany 2011
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